Fig 5. Effects of siRNA-induced multiple knockdown of tight junction proteins on transepithelial electrical resistance (TER) in HK-2 cell monolayers.

TER was significantly decreased by claudin-2/ZO-1 siRNA cotransfection, but significantly increased by occluding/claudin-2 siRNA co-transfection. When claudin-2, occludin, and ZO-1 were simultaneously inhibited, TER was suppressed. Data are mean ± SD. *P<0.05 vs. vehicle; by Student’s t-test for unpaired data.