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Protein sequence alignment of wild type and mutant ovine fibroblast growth factor 14, highlighting the Q16X mutation which induces a stop codon. The Q16X mutation was determined after whole genome sequencing using Illumina HiSeq 2000 machine, aligning to the reference sheep genome (Oar3.1) using BWA-MEM, calling variants with GATK 3.4–46, and predicting variant effects with SnpEff 4.1.
