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(A) Substrate saturation curve of CobB at different Acs concentrations. (B) Acs and Acs K609A mutant deacetylation (%). (C) Western blot with anti-Ac-K of Acs (lanes 1 and 2) and Acs K609A mutant (lanes 3 and 4) before (control) and after CobB deacetylation (deacetylated). (D) Number of lysines deacetylated by CobB per Acs molecule at different Acs concentrations.
