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. 2017 Dec 18;12(12):e0189903. doi: 10.1371/journal.pone.0189903

Fig 1. Tube formation assay.

Fig 1

Angiogenic capacity analyzed by tube formation of human endothelial cells treated with different PD fluids. Automated analysis performed with CellProfiler Software showing (A) mean area and number of closed areas by endothelial cell branches (B) and (C) illustrative example of methodology (left image: red lines mark endothelial cells and their branches; right image: areas framed by endothelial cells are shown in different colors). Representative examples of the tubular network formed with BPDF and LPDF from 4 independent experiments performed in triplicates are given in (D). *p<0.05; **p<0.01; ****p<0.0001 for BPDF vs. all other; #p<0.05 BPDF vs. LPDF.