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. 2017 Dec 18;12(12):e0189062. doi: 10.1371/journal.pone.0189062

Fig 3. CaMV TAV and TAVm3 proteins expressed in turnip plants and A. thaliana in a viral or non-viral context.

Fig 3

(A and B) Transient expression of EGFP-TAV and EGFP-TAVm3 in turnip epidermal cells bombarded with the corresponding pCK-EGFP recombinant plasmids (A, panels 1 and 2) and in A. thaliana (ecotype Columbia) protoplasts transfected by PEG with same constructions (B, panels 1 and 2). Cells were observed 16 h after transfection, by LSCM. (C) Immunolocalisation of TAV and TAVm3 (panels 1 and 2) in protoplasts obtained from transgenic A. thaliana (ecotype Columbia) lines expressing wild-type or TAVm3 [12]. Protoplasts were incubated with polyclonal anti-TAV antibodies [38] and with anti-rabbit IgG secondary antibodies conjugated to green fluorochrome Alexa 488. (D) Detection of TAV proteins by EGFP fluorescence, 21 dpi, in turnip epidermal cells from systemic leaves infected with wild-type CaMV (panels 1 and 2) or CaMV-TAVm3 (panel 3) through interactions with ectopic EGFP-TAV (panel 1) and EGFP-TAVm3 (panels 2 and 3) expressed upon bombardment with the corresponding plasmids. Immunofluorescence detection of TAV and TAVm3 in protoplasts isolated from CaMV-TAV and CaMV-TAVm3-infected turnip leaves (panels 4 and 5). The LSCM settings and acquisition conditions of the images were identical in all panels. Images in (A), (B), (C) and (D) (panels 1–3) are projections; those in (D) (panels 4–5) show single sections. Scale bars (A and D): 10 μm, (B and C): 5 μm.