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. Author manuscript; available in PMC: 2017 Dec 18.
Published in final edited form as: Nat Methods. 2016 Oct 10;13(11):953–958. doi: 10.1038/nmeth.4028

Figure 1.

Figure 1

Simultaneous, single-tube sequencing of DNA and RNA. (a) Schematic of Simul-seq method. (b) Cross-species mapping rates for Simul-seq libraries produced from a mixture of yeast mRNA and human genomic DNA (n = 2) as well as yeast RNA-seq (n = 3) and human DNA-seq controls (n = 2). (c) Droplet digital PCR (ddPCR) assays on Simul-seq libraries (n = 3 technical replicates per library) with varying amounts of RNA-specific PCR amplification followed by an additional five cycles of PCR with primer sets for both RNA and DNA. (d) DNA and RNA library ratios measured by ddPCR (n = 3 technical replicates per library) are correlated with subsequent read ratios.