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. Author manuscript; available in PMC: 2017 Dec 18.
Published in final edited form as: Nat Methods. 2016 Oct 10;13(11):953–958. doi: 10.1038/nmeth.4028

Figure 5.

Figure 5

Identification and biochemical characterization of a recurrent mutation in KIF3B. (a) Schematic of KIF3B locus, including positions of mutations found in targeted resequencing of 49 esophageal adenocarcinoma patients and 25 paired controls. Coverage for representative sample is shown. Note, the original sample that was subjected to the Simul-seq protocol was included as a positive control. (b) Strip plot of ATPase activity (n = 2) for recombinant wild-type and R293W mutant KIF3B motor domains when incubated with increasing quantities of microtubules. Activity was quantitated using an endpoint measurement of free phosphate.