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. Author manuscript; available in PMC: 2017 Dec 18.
Published in final edited form as: Methods Mol Biol. 2016;1474:291–307. doi: 10.1007/978-1-4939-6352-2_19

Fig. 2.

Fig. 2

Immunogold labeling of collagen VII in a cryosection of bovine tongue. The primary antibody was a mouse monoclonal against collagen VII prepared in the Jones lab. The secondary antibody was an AuroProbe goat anti-mouse IgG conjugated with 5 nm gold (GE Healthcare UK, Ltd.). Note the gold particles localizing collagen VII to the region beneath the hemidesmosomes (HD) and the basement membrane (BM) at the point of interaction of so-called anchoring fibrils with the BM and also in plaque-like aggregates in the dermis (arrows). Bar: 200 nm