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. Author manuscript; available in PMC: 2017 Dec 18.
Published in final edited form as: Nature. 2017 Mar 29;544(7650):316–321. doi: 10.1038/nature21720

Figure 1. Inhibiting postsynaptic expression of both Syt1 and Syt7 blocks LTP by a cell-autonomous mechanism.

Figure 1

a, Experimental strategy. Left, schematic of stereotactic virus injections; center, representative EGFP-fluorescence images of hippocampal slices after global AAV or sparse lentivirus infections; right, schematic of whole-cell recordings in acute slices.

b, AAV-mediated double knockdown (DKD) of both Syt1 and Syt7 suppresses NMDAR-dependent LTP. Left, representative traces and LTP time course; right, summary graphs of LTP magnitude.

c, Same as b, but for sparse lentiviral DKD of Syt1 and Syt7.

Data are means ± SEM (numbers in bars = number of neurons/mice analyzed). Statistical significance was assessed by Kruskal-Wallis ANOVA followed by the Mann-Whitney U test (c), or by only the Mann-Whitney U test (d; **, p<0.01; ***, p<0.001). In b and c, calibration bars = 50 pA, 50 ms.