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. Author manuscript; available in PMC: 2017 Dec 18.
Published in final edited form as: Nature. 2017 Mar 29;544(7650):316–321. doi: 10.1038/nature21720

Figure 2. Inhibiting postsynaptic Syt1,7 expression by diverse molecular manipulations universally blocks LTP.

Figure 2

a & b, Blocked LTP after Syt1,7 DKD is rescued by shRNA-resistant WT (a) but not mutant Syt7 (b; Syt7-C2A* contains inactivated C2A-domain Ca2+-binding sites). Left, representative traces and LTP time course; right, summary graphs of LTP magnitude.

c, Conditional KO (cKO) of postsynaptic Syt1 in constitutive Syt7 KO mice blocks LTP.

d, Postsynaptic Syt1,7 DKD also blocks LTP induced by depolarizing voltage pulses in the presence of the NMDAR-antagonist APV.

Data are means ± SEM (numbers in bars = number of neurons/mice analyzed). Statistical significance was assessed in a–c with Kruskal-Wallis ANOVA followed by pairwise comparisons with the Mann-Whitney U test (a–c), or simply by the Mann-Whitney U test (d, e; *, p<0.05; **, p<0.01; ***, p<0.001). Calibration bars = 50 pA, 50 ms for a–d; 50 pA, 10 ms for e.