Extended Figure 8.

a, Schematic representation of the behavioral paradigm used to assess the impact of DUSP6 downregulation in the vmPFC of females. Behavioral consequences of DUSP6 downregulation in female vmPFC in the b, forced swim test (FST), c, open field test (OFT), and d, elevated plus maze (EPM). e, Schematic representation of the behavioral paradigm used to assess the impact of DUSP6 overexpression in females following sub-chronic variable stress. Bottom graph shows the HSV-mediated overexpression of DUSP6 covering the infra- and prelimbic region of the vmPFC in mice. Behavioral consequences of DUSP6 viral overexpression in the f, novelty-supressed feeding test (NSF) and g, sucrose preference test. Significance in tests e was determined using independent sample t-test while significance in b–d and f–g was determined using two-way ANOVA with Tuckey correction. b–d n =7/condition, e n=5/condition, f–g n=10/condition. Bars, mean ± sem; * p<0.05.