Fig. 2. Fexaramine (Fexa) inhibits bone resorption. (A) Fexa (5 µM) was added during the indicated culture days in the presence of macrophage colony-stimulating factor (M-CSF) (30 ng/mL) and receptor activator of nuclear factor-κB ligand (RANKL) (100 ng/mL). (B) Bone marrow-derived macrophages (BMMs) from farnesoid X receptor (FXR)^+/+ and FXR^−/−mice were cultured with M-CSF (30 ng/mL) and RANKL (100 ng/mL) in the presence of indicated concentrations of fexa for 4 days, and tartrate-resistant acid phosphatase-positive (TRAP⁺) osteoclasts were counted. (C) BMMs were differentiated on dentine slices with M-CSF (30 ng/mL) and RANKL (100 ng/mL) for four days and fexa (5 µM) was treated for an additional two days. The number of resorption pits were counted. Scale bar=200 µm. Data are expressed as mean±standard deviation from at least three independent experiments. ^*P<0.05. MNCs, multinucleated osteoclasts; WT, wild-type; Veh, vehicle.