Fig. 4. Fexaramine (Fexa) inhibits receptor activator of nuclear factor-κB ligand (RANKL)-induced signaling pathways. (A-C) Bone marrow-derived macrophages (BMMs) were preincubated in the absence or presence of fexa (5 µM) for 30 min, and then treated with or without 200 ng/mL RANKL for (A, B) 15 min or (C) 24 hr. Cell lysates were then subjected to Western blotting analysis with the indicated antibodies. Data are expressed as the mean±standard deviation from at least three independent experiments. ^*P<0.05. Veh, vehicle; p-p38, phospho-p38; ERK, extracellular signal-regulated kinase; p-ERK, phosphor-extracellular signal-regulated kinase; p-GSK, phosphor-glycogen synthase kinase.