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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: FEBS J. 2017 Nov 13;284(24):4216–4232. doi: 10.1111/febs.14301

Table 1. Selection of published interactions between NuRD subunits.

The interactions were classified as ‘high confidence’ (high resolution structural data and/or biophysical characterization), ‘low confidence, folding uncertainty’ (when one or both of the interacting proteins are probably not properly folded), or ‘low confidence, bridging uncertainty’ (when the observed interaction is likely to be indirect and is observed because of bridging by endogenous components). In the case of immunoprecipitations and pulldowns, ‘Protein 1’ is the bait, and ‘Protein 2’ the prey. 35S: SDS-PAGE and detection of 35S-labelled proteins; WB: western blot; Dm: Drosophila melanogaster.

Protein 1 Protein 2 Expression System Method/Detection Reference
High confidence
MBD2/3 GATAD2A E. coli NMR/ITC [16, 42]
HDAC1 MTA1 HEK293 cells X-ray [15]
RBBP4 MTA1 Sf9 cells/HEK293 cells X-ray [17, 19]
Low confidence, folding uncertainty
MBD3 MTA2 E. coli/IVT GST Pulldown/35S [37]
MBD3 HDAC1 E. coli/Sf9 cells GST Pulldown/WB [37]
MBD3 RBBP4/7 E. coli GST Pulldown/WB [37]
MBD3 GATAD2B E. coli/IVT GST Pulldown/35S [43]
HDAC1 HDAC2 E. coli/IVT GST Pulldown/35S [30]
HDAC1 CHD4 Sf9 cells/IVT FLAG Pulldown/35S [44]
MBD2/3 GATAD2A/B HEK293 cells/E. coli/IVT GST Pulldown/WB/35S [45]
MBD3 MTA2 E. coli GST Pulldown/WB [21]
MBD3 HDAC1 E. coli GST Pulldown/WB [21]
MBD3 MBD2 E. coli GST Pulldown/WB [20]
Dm. MBD2/3 Dm. p55 (RBBP-like) E. coli/IVT GST Pulldown/35S [46]
Dm. MBD2/3 Dm. MI-2 (CHD4-like) E. coli/IVT GST Pulldown/35S [46]
Low confidence, bridging uncertainty
MBD2 RBBP7 HEK293 cells FLAG Pulldown/WB [34]
MBD2 MTA2 HEK293 cells FLAG Pulldown/WB [34]
MBD2 HDAC2 HEK293 cells FLAG Pulldown/WB [34]
MBD3 GATAD2A/B HEK293 cells GST Pulldown/WB [29]
HDAC2 CHD4 HeLa cells Co-IP/WB [47]
HDAC1 RBBP4 SAOS cells Co-IP/WB [48]
HDAC1 GATAD2A/B HEK293 cells GST Pulldown/WB [29]
RBBP4/7 GATAD2A/B HEK293 cells GST Pulldown/WB [29]
MTA1 CHD4 HeLa cells Co-IP/WB [49]
MTA1 HDAC2 HeLa cells Co-IP/WB [49]
MTA1 MBD3 HeLa cells Co-IP/WB [49]
Dm. p66-like Dm. MI-2 (CHD4-like) Dm. embryos Co-IP/WB [50]