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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Biomaterials. 2017 Oct 26;154:60–73. doi: 10.1016/j.biomaterials.2017.10.038

Figure 3.

Figure 3

Optimization of the BLT Sandwich culture system using mouse SI organoids under different culture settings for 4 days. A: Sandwich configuration composed of Col IV thin coating (100 μg/mL) and Col I gel overlay synergistically enhances the expansion of Lgr5+ cells. Representative confocal images (A) show the morphology and presence of Lgr5-GFP in IEC monolayer on transwells under different culture settings with or without Col IV coating and Col I overlay. B1–2: Col IV coating concentration dictates the number of Lgr5-GFP+ cells (B1, normalized to no Col IV coating control) and the percentage of Lgr5/Ki67 co-positive population (B2). n = 3–4 wells, data represents mean ± SD from one representative trial out of three independent trials using SI organoids derived from three donor mice.