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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Biomaterials. 2017 Oct 26;154:60–73. doi: 10.1016/j.biomaterials.2017.10.038

Figure 4.

Figure 4

Characterization of the BLT Sandwich culture system. A: Representative phase contrast and fluorescence images showing the proliferation and presence of Lgr5-GFP of IEC monolayer at day 4. Contour of epithelial island/monolayer is delineated by dashed lines in phase contrast images for better visualization. Zoomed-in view depicts the undulating pattern of IEC monolayer/sheet that is closely associated with Lgr5-GFP+ cells. B1: Confocal images comparing the expression of Lgr5 and Ki67 between 2D Sandwich and 3D Matrigel culture systems at day 4 (a); B2–3: Flow cytometry results comparing the level of Ki67+ cells (B2) and Lgr5/Ki67 co-positive cells (B3) between the two culture systems. Mean ± SEM, averaged from n = 3–4 independent trials with different SI organoid donor mice.

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