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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Biomaterials. 2017 Oct 26;154:60–73. doi: 10.1016/j.biomaterials.2017.10.038

Figure 5.

Figure 5

Validation of the multi-lineage differentiation potential of BLT Sandwich culture-expanded ISCs. A1–4: qPCR analysis of relative mRNA expression of Lgr5 and mature IEC markers (Alpi, Lyz1, Muc2 and ChgA) from monolayer IECs (with ~35% baseline Lgr5-GFP level) treated with indicated differentiation cocktails for 3 days with ENR being present in all conditions. mRNA levels of target genes were normalized to ENR-alone conditions. Mean ± SEM, n = 3 independent trials each with 2–3 biological repeats. **, ***: significantly different versus ENR-alone control. B: Colorimetric staining (Alpi) and immunocytochemistry staining of mature IEC lineage markers after 3-day differentiation. Representative images from three independent trials with n = 2 wells (8–10 images) each condition. Scale bars for all Alpi images: 500 μm.