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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Biomaterials. 2017 Oct 26;154:60–73. doi: 10.1016/j.biomaterials.2017.10.038

Figure 7.

Figure 7

Positive effect of LDN-193189 (L) on the expansion of Lgr5-GFP+ cells cultured from freshly isolated mouse SI crypts for 4 days in 2D BLT Sandwich culture under different culture media conditions. A: Number of Lgr5-GFP+ cells normalized to the control without LDN in response to different concentrations of LDN in ISC maintenance media (i.e. ENR+CV). B: Percentage of resulting Lgr5-GFP+ cells under different media conditions; C: Normalized number of Lgr5-GFP+ cells (to ENR-alone control) under different conditions with or without CV, LDN (200 nM) and Noggin (N). Data represents mean ± SEM, n = 3–4 independent trials with different donor mice.