Fig. 3.

MA delivery of a rAdHu5 vector encoding HIV-1 CN54 gag induces systemic and mucosal CD8⁺ T cell memory responses. (A) Female C57BL/6 mice were immunized with rAdHu5-CN54 gag virus by dried MA application to dorsal skin or left naïve. Blood and genital tract tissues were collected 28 days post immunization for flow cytometric analysis. Frequency of Db/CN54gag tetramer expressing cells in blood (B) and genital tract (D) induced by MA immunization is shown from a gated CD45⁺ CD8⁺ population. Values (B and D) represent percentage of cells in each gate and are representative of four naïve and nine vaccinated mice from two independent experiments. (C and E); Bar graphs represent the frequency of Db/CN54gag Tetramer⁺ CD8⁺ T cells in blood (C) and genital tract (E) of rAdHu5-CN54 gag MA immunized mice.