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. 2017 Dec 28;268:166–175. doi: 10.1016/j.jconrel.2017.10.026

Fig. 4.

Fig. 4

Memory CD8+ T cells elicited by MA vaccination express peripheral tissue homing and retention and markers in the female genital tract. (A) Db/CN54gag tetramer+ CD8+ T-cells were assessed in the blood and genital tracts of female B6 mice 28 days post immunization with rAdHu5-CN54gag MA. (B) Dot plots of Db/CN54gag-specific CD8+ T cells in blood (lower panel) and genital tracts (upper panel) 28 days after MA immunization with rAdHu5-CN54 gag. (C) Representative histograms indicating the frequency of CD8+ T cell differentiation/memory markers (KLRG1, CD127 and CD62L) expressed by Db/CN54gag tetramer+ CD8+ T cells (red lines) isolated from blood (lower panels) or genital tracts (upper panels) of rAdHu5-CN54 gag MA immunized mice. Grey histograms represent isotype control staining. (D) Representative histograms indicating the frequency of CD103, CD49a, CD69 or CXCR3 expressing Db/CN54gag tetramer+ CD8+ T cells (red lines) isolated from blood (lower panels) or genital tracts (upper panels) of rAdHu5-CN54gag MA immunized mice. Grey histograms represent staining with isotype controls. Data are representative of four naïve and nine vaccinated mice from two independent experiments.