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. 2017 Dec 28;268:166–175. doi: 10.1016/j.jconrel.2017.10.026

Fig. 6.

Fig. 6

rAdHu5-CN54gag MA immunization generates long-lived, antigen-specific, tissue resident memory CD8+ T cells within female genital tract tissues. (A) Schematic of the experimental design to assess Db/CN54gag tetramer+ CD8+ T-cells in blood and genital tract of female mice 365 days after MA immunization with rAdHu5-CN54gag. (B) Bar graph indicates the frequency of Db/CN54gag tetramer+ CD8+ cells (mean ± SEM) enumerated in the blood and isolated genital tract tissue of naïve and rAdHu5-CN54gag MA immunized mice after 365 days. (C) Representative dot plots of Db/CN54gag tetramer+ CD8+ T cells assessed from blood (upper panels) and isolated genital tract tissue (lower panels) from mice enumerated in (B). (D) Representative contour plots showing the expression of KLRG1 and CD62L or CD103 and CD69 among Db/CN54gag tetramer+ CD8+ T cells isolated from blood (upper panels) or genital tract tissue (lower panels) of mice immunized with rAdHu5-CN54gag MA 365 days prior to analysis. (E) Representative histograms indicating the frequency of CD127, CXCR3 or CD49a expressing Db/CN54gag tetramer+ CD8+ T cells (red histograms) isolated from blood (upper panels) or genital tract (lower panels) of mice immunized with rAdHu5-CN54gag MA 365 days prior to analysis. Grey histograms represent staining with isotype control. Data are from one experiment (n = 4/group).