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. 2017 Dec 28;268:166–175. doi: 10.1016/j.jconrel.2017.10.026

Fig. 7.

Fig. 7

rAdHu5-CN54gag MA immunization generates long-lived, antigen-specific, tissue resident memory CD8+ T cells within the respiratory tract. (A) Schematic of the experimental design to assess Db/CN54gag tetramer+ CD8+ T-cells in BAL and lung parenchyma 365 days after MA immunization with rAdHu5-CN54gag. (B) Flow cytometric analysis showing Db/CN54gag tetramer+ cells among CD45+ CD8+ T cells isolated from BAL of naïve or MA immunized mice. (C) Bar graph indicates the frequency of Db/CN54gag tetramer+ CD8+ cells (mean ± SEM) enumerated in lung tissue of naïve and rAdHu5-CN54 gag MA immunized mice after 365 days. (D) Lung tissues isolated from naïve mice or rAdHu5-CN54gag MA immunized mice were assessed after 365 days for infiltration of Db/CN54gag tetramer+ CD8+ T cells by flow cytometry. (E) Representative contour plots showing the expression of KLRG1 and CD62L (left) or CD103 and CD69 (right) among Db/CN54gag tetramer+ CD8+ T cells isolated from lung tissues of mice immunized with rAdHu5-CN54gag MA 365 days prior to analyses. (F) Representative histograms indicating the frequency of CD127, CXCR3 or CD49a expressing Db/CN54gag tetramer+ CD8+ T cells (red histograms) isolated from lungs of mice immunized with rAdHu5-CN54gag MA 365 days prior to analysis. Grey histograms represent staining with isotype controls. Data are from one experiment (n = 4/group).