Table 2.
Characteristics of in silico predicted human leukocyte antigen–DRB1*0101-restricted 15 mer epitopes derived from shortlisted antigenic proteins of Leishmania donovani.
| Sl. no. | Protein-Id | Peptide | Position | SYFPEITHI | IEDB | NETMHC-II |
|---|---|---|---|---|---|---|
| 1 | XP_003865112.1 | QSKFRPISASSMPDE | 231 | 34 | 1.15 | SB |
| IQSYRLLKDAVASPL | 274 | 29 | 1.15 | SB | ||
| LQSFVVTCSAAALTL | 431 | 25 | 0.19 | SB | ||
| QSFVVTCSAAALTLA | 432 | 25 | 0.47 | SB | ||
| ALLQSFVVTCSAAAL | 429 | 21 | 1.33 | SB | ||
| 2 | XP_003862935.1 | ILDVIFMTSRLVAKM | 522 | 30 | 2.05 | SB |
| LRRLFSIRTNALARE | 173 | 28 | 2.74 | SB | ||
| FDLFLFSNGAVVWWG | 282 | 27 | 0.32 | SB | ||
| TLGFQPLAVEPALDR | 353 | 26 | 0.71 | SB | ||
| NCFDLFLFSNGAVVW | 280 | 25 | 1.06 | SB | ||
| RVRLRRLFSIRTNAL | 170 | 24 | 0.77 | SB | ||
| 3 | XP_003863771.1 | LLALILLGGIGAVGY | 563 | 36 | 1.33 | SB |
| YPVYPFLASNAALLN | 312 | 33 | 0.14 | SB | ||
| LPSFHAMSAFHSAAK | 399 | 32 | 0.6 | SB | ||
| NAALLNLIPSLLYRV | 321 | 30 | 0.6 | SB | ||
| VYPFLASNAALLNLI | 314 | 26 | 0.09 | SB | ||
| RALLALILLGGIGAV | 561 | 25 | 2.05 | SB | ||
| AALLNLIPSLLYRVQ | 322 | 22 | 0.6 | SB | ||
| 4 | XP_003858976.1 | LQVFTAICASFAHGA | 332 | 33 | 2.27 | SB |
| FPFFSGVAPIVASWF | 145 | 30 | 0.71 | SB | ||
| ERVFRYLQVFTAICA | 326 | 28 | 2.74 | SB | ||
| KDDFPFFSGVAPIVA | 142 | 27 | 2.18 | SB | ||
| DFPFFSGVAPIVASW | 144 | 26 | 0.62 | SB | ||
| LESFFVLFKGASKRL | 204 | 26 | 2.46 | SB | ||
| FFVLFKGASKRLKWS | 207 | 25 | 2.09 | SB | ||
| FRYLQVFTAICASFA | 329 | 24 | 2.09 | SB | ||
| SRGFSAELSAALVVS | 411 | 24 | 1.06 | SB | ||
| RGFSAELSAALVVSF | 412 | 24 | 1.36 | SB | ||
| 5 | XP_003857910.1 | LALLIMLYALIATQF | 115 | 33 | 0.28 | SB |
| LIMLYALIATQFSDD | 118 | 33 | 0.96 | SB | ||
| IMLYALIATQFSDDA | 119 | 31 | 2.51 | SB | ||
| VSVLALLIMLYALIA | 112 | 25 | 0.28 | SB | ||
| VLALLIMLYALIATQ | 114 | 25 | 0.28 | SB | ||
| 6 | XP_003858984.1 | VSILRQLLSVTAHTH | 383 | 32 | 0.77 | SB |
| KVAVSILRQLLSVTA | 380 | 27 | 0.97 | SB | ||
| GISFSRAFAANIESA | 127 | 26 | 2.82 | SB | ||
| YQFYHRARSYVIFTT | 692 | 24 | 0.09 | SB | ||
The consensus peptides with binding scores above 21 for SYFPEITHI less than 3 for IEDB and predicted IC50 ≤ 50 nM (Strong Binding) for NETMHC-II were selected for further analysis.