Fig. 3.

Both cytoplasmic and nuclear-localized XopH variants are biologically active. a Confocal laser scanning microscopy of N. benthamiana leaves two dpi with Agrobacterium-mediating expression of GFP, XopH, or XopH fused to a nuclear localization signal (NLS), a nuclear export signal (NES), and a mutated NES (nes), respectively. DAPI (4′,6′-Diamidino-2-phenylindole) staining indicates nuclei. b HR induction by XopH and derivatives. The same Agrobacterium strains analyzed in a were infiltrated into leaves of resistant pepper ECW-70R plants. Leaves were harvested two dpi and bleached with ethanol. c Agrobacterium-mediated expression of His₆-tagged GFP fusion proteins. Samples were harvested two dpi from the same areas as investigated in a and analyzed by immunoblot. Signals at expected sizes are marked by an arrow. d SAX-HPLC profiles of extracts from [³H]-myo-inositol-labeled transgenic N. benthamiana seedlings that were inoculated with the Agrobacterium strains described in a. The experiments were repeated twice (a–c) and once (d), respectively, with similar results