Fig. 4.

XopH hydrolyzes InsP₆ to InsP₅ [1/3-OH]. a Structure and symmetry of myo-inositol. b InsP₆ (10 nmol) was incubated with XopH or XopH_CH (catalytically dead). Reaction products were separated by PAGE and stained. InsP₆ and InsP₅ isomers (10 nmol) served as controls. Orange G, loading dye. The experiment was repeated twice with similar results. c Stacked ion chromatograms of InsP₆ (2 nmol), XopH (0.13 µg/µL) + InsP₆ (2 nmol), a phosphate standard (35 nmol) and all InsP₅ isomers (2 nmol each) eluted on CarboPac PA100: eluent: 0.5 M HCl and water (18 MΩ * cm); detection: post-column reaction with Fe^{3 +}, UV, 290 nm, injection volume: 10 µL