Skip to main content
. 2017 Dec 18;8:2159. doi: 10.1038/s41467-017-02195-8

Fig. 4.

Fig. 4

XopH hydrolyzes InsP6 to InsP5 [1/3-OH]. a Structure and symmetry of myo-inositol. b InsP6 (10 nmol) was incubated with XopH or XopHCH (catalytically dead). Reaction products were separated by PAGE and stained. InsP6 and InsP5 isomers (10 nmol) served as controls. Orange G, loading dye. The experiment was repeated twice with similar results. c Stacked ion chromatograms of InsP6 (2 nmol), XopH (0.13 µg/µL) + InsP6 (2 nmol), a phosphate standard (35 nmol) and all InsP5 isomers (2 nmol each) eluted on CarboPac PA100: eluent: 0.5 M HCl and water (18 MΩ * cm); detection: post-column reaction with Fe3 +, UV, 290 nm, injection volume: 10 µL