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. 2017 Dec 18;8:2159. doi: 10.1038/s41467-017-02195-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — XopH phytase activity affects plant hormone pathways. a qRT-PCR analysis of N. benthamiana leaves transiently expressing XopH and mutant derivatives, untreated or 20 min post wounding. ev empty T-DNA. Asterisks indicate statistically significant differences to corresponding ev samples (Mann–Whitney test; *p < 0,05; **p < 0,01; ***p < 0,001). b Protein expression two dpi in the same plants analyzed in a. Immunoblot signals at expected sizes are marked by asterisks. c qRT-PCR analysis of COI1-, EIN2-, and EBF1-silenced N. benthamiana plants (siCOI1, siEIN2, and siEBF1) transiently expressing XopH and its catalytically inactive variant (CH), respectively. GFP-silenced plants (siGFP) served as control. Asterisks indicate statistically significant differences (Student’s t test; *p < 0,05; **p < 0,01). d Silencing efficiencies determined by qRT-PCR. Values indicate percent reduction of COI1, EIN2, and EBF1 transcript levels in the respective silenced plants, relative to GFP-silenced plants, both expressing inactive XopH_CH. Combined data from three independent experiments (with three biological replicates per experiment) are shown (a, c, d). Error bars indicate s.e.m.