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. 2017 Dec 18;7:17702. doi: 10.1038/s41598-017-17377-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantified GAP-43 protein expression in the brain. (A) Near-infrared (NIF) western blots normalized to GAPDH show GAP-43 protein in CD and non-CD brains at each time point after PTZ induced seizures. Western blot image was cropped to remove protein ladder. (B) GAP-43 protein in the brain as measured by ELISA in CD rats compared to non-CD rats at each time point. (C) ELISA results show a higher level of GAP-43 protein in CD rats as compared to non-CD rats 120 days after PTZ induced acute seizures. Error bars represent standard error of the mean. Sample sizes: Non-CD + Saline n = 5, + PTZ Day 2 n = 4, + PTZ Day 15 n = 4, + PTZ Day 30 n = 4. CD + Saline n = 4, + PTZ Day 2 n = 4, + PTZ Day 15 n = 4, + PTZ Day 30 n = 4.