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. 2017 Dec 14;11:386. doi: 10.3389/fncel.2017.00386

Figure 1.

Figure 1

HIF-1α mRNA and protein expression levels in cultured primary human astrocytes following hypoxia or hypoxia+hypothermia. Data shown include mean fold change in expression (± S.E.M.) of cells treated with 5% oxygen for 6 h with no temperature change (Hypoxia) or with 32°C hypothermic intervention after initial incubation for 2 h of hypoxia for a total of 6 h (Hypoxia + Hypothermia) compared with untreated normoxic, normothermic astrocytes (Control). Each bar represents the Mean ± S.E.M. for each of the conditions. Kruskall-Wallis with Conover-Inman post-hoc analysis tests were used to identify significant differences between samples using StatDirect 3 software. *Represents statistical significance (p < 0.001). (A) Shows RT-qPCR data (N = 5) using Taqman probes and 2∧−ΔΔCT analysis normalized to the two housekeeping genes, PPIA and CDKN1B. (B) Shows Proteome Profiling Array data (N = 3). FC, Fold change.