Table 3.

Fold regulation comparison for significant proteins within human p53 and apoptosis signaling and related pathways and their corresponding gene expression from the microarray data analysis.

Target name	Gene fold change ± S.E.M.	Protein fold change ± S.E.M.
Bax	0.15* ± 0.03	0.43* ± 0.07
Bcl-x	0.24* ± 0.21	0.36* ± 0.17
Cleaved Caspase-3	–	0.73* ± 0.12
cIAP-2	NS	0.55* ± 0.07
Claspin	0.32* ± 0.27	0.84* ± 0.08
Clusterin	NS	0.56* ± 0.19
FADD	0.11* ± 0.05	0.82* ± 0.05
HIF-1α	0.06* ± 0.01	0.79* ± 0.02
HO-1/HMOX1/HSP32	0.38* ± 0.03	0.54* ± 0.05
HSP27	0.38* ± 0.02	0.78* ± 0.13
HSP70	0.4* ± 0.01	0.71* ± 0.1
HTRA2/Omi	0.35* ± 0.06	0.49* ± 0.19
Livin	2.17* ± 0.02	0.47* ± 0.09
p21/CIP1/CDKN1A	NS	0.45* ± 0.21
p27/Kip1	0.35* ± 0.03	0.59* ± 0.27
Phospho-p53 (S15)	0.43* ± 0.01	0.67* ± 0.14
Phospho-Rad17 (S635)	–	0.65* ± 0.02
SMAC/Diablo	NS	0.60* ± 0.25
Survivin	5.77* ± 0.49	1.41* ± 0.12

35 human apoptosis-related proteins closely associated with the Wnt signaling pathway were investigated using a Proteome Profiling array. Data shown include mean fold change (± S.E.M.) of significantly altered proteins in astrocyte cell lysates treated with 5% O₂ for 6 h with no temperature change (hypoxia) or with a 32°C hypothermic intervention after initial incubation for 2 h of hypoxia (hypoxia+hypothermia; for a total of 6 h). Fold change (FC) was obtained by dividing the mean of the expression level in hypoxia+hypothermia by the mean of its corresponding hypoxia expression level. The data represent a comparative analysis for selected significantly expressed proteins (normalized to array control) and their corresponding genes (normalized to array signal) and in hypoxia+hypothermia and hypoxia. The results are presented as mean ± S.E.M. for microarray analysis (n = 2) and proteome profiler analysis (n = 3). Kruskal-Wallis with Conover-Inman post hoc analysis was used to identify significant differences between samples (

^*P < 0.05, NS = not significant, – not detected in the microarray analysis).