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. 2017 Dec 14;8:1011. doi: 10.3389/fphys.2017.01011

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Copyright © 2017 Pecoraro, Marino, Salvaggio, Capparucci, Di Caro, Iaria, Salvo, Rotondo, Tibullo, Guerriero, Scalisi, Zimbone, Impellizzeri and Brundo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mRNA gene expression of MT1 in zebrafish after exposure to Ag free nanoparticles. Bars represent the mean ± SEM of three independent experiments. Statistical analysis was carried out by ANOVA test. A p-value of less than 0.05 (p < 0.05) was accepted as statistically significant between experimental and control groups. (Calculated value of 2^−ΔΔCt in untreated sample was 1).