Fig. 3.

CYP92B14 biosynthesizes (+)-sesamolin and (+)-sesaminol from (+)-sesamin in vitro. a Microsome fractions prepared from a series of transformed yeasts were subjected to enzyme assays using (+)-sesamin as a substrate. a: (+)-sesaminol; b: (+)-sesamin; c: (+)-sesamolin. b CO-difference spectra of microsome fractions expressing CYP92B14. c Expression of recombinant CYP92B14 proteins in yeasts was immunologically detected with an anti-CYP92B14 antibody raised against the peptide sequence that is common to full-length CYP92B14 and Del4C