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. 2017 Dec 14;8:661. doi: 10.3389/fneur.2017.00661

Figure 11.

Figure 11

Figure 11

α-Asarone treatment reduces phosphorylation of IκB-α and IKK-β, and thus reduces the available p-IκB-α p-IKK-β kinase for degradation in primary microglia stimulated with LPS. Experiments were performed as previously described. (A) LPS exposure induces phosphorylation of IκB-α and IKK-β activation followed by degradation, as assessed by antibodies against phospho-IκB-α, IκB-α, phospho-IKK-β, and IKK-β at different time points. (B) After treatment with α-asarone for 1 h, primary rat microglia were stimulated with LPS (1 µg/ml) for 3 h, the protein level were analyzed by Western blot analysis. The p-IκB-α or IκB-α/GAPDH and the p-IKK-β or IKK-β/GAPDH ratio were determined by densitometry analysis. Con, control group; LPS, lipopolysaccharide-treated model group; LPS-100, LPS-50, LPS-25, microglial pretreatment with different concentrations of α-asarone (100, 50, and 25 µg/ml) group; IκB-α, inhibitor kappa B-alpha; IKK-β, I kappaB kinase-β. x ± s from three independent experiments. *p < 0.05, **p < 0.01, vs. Con group; #p < 0.05, ##p < 0.01, vs. LPS group.