Figure 12.

The effect of α-asarone on the iNOS and COX-2 protein expression levels in LPS-stimulated primary microglia. After treatment with α-asarone for 1 h, primary rat microglia were stimulated with LPS (1 µg/ml) for 3 h. The protein level of iNOS (A) and COX-2 (B) were analyzed by Western blot analysis. Quantitative data are displayed on each panel, respectively. Con, control group; LPS, lipopolysaccharide-treated model group; LPS-100, LPS-50, LPS-25, microglial pretreatment with different concentrations of α-asarone (100, 50, and 25 μg/ml) group; iNOS, including nitric oxide synthase; COX-2, cyclooxygenase-2. x ± s from three independent experiments. *p < 0.05, **p < 0.01, vs. Con group; ^#p < 0.05, ^##p < 0.01, vs. LPS group.