Fig. 2.

Knockdown of ANGPTL8 potentiates TNFα-induced NF-κB activation. a Efficacy of three different ANGPTL8-RNAi plasmids on the transcription (upper panels) and expression (lower panels) of ANGPTL8 in the control or shANGPTL8 stable HepG2 cell lines (n = 3). b Effects of ANGPTL8-RNAi on TNFα-induced NF-κB activation in the cell lines indicated as in a (n = 3). c Effects of ANGPTL8-RNAi on IFNγ (100 ng/mL) induced IRF1 activation (n = 3). d Effects of ANGPTL8-RNAi on TNFα-induced IL8, CXCL2 and NFKBIA transcription for 2 h in cell lines indicated as in a (n = 3). e Effects of ANGPTL8-RNAi on TNFα-induced IKK and IκBα phosphorylation in ANGPTL8-RNAi-#3 or control cells in a. Data are shown as the mean ± SEM, unpaired two-tailed Student’s test was used for statistics (a–d), *p < 0.05, **p < 0.01, NS > 0.05. Data are representative of three independent experiments