Fig. 3.

Knockout of ANGPTL8 potentiates TNFα-induced NF-κB activation. a Genotyping of ANGPTL8 ^−/− and wild-type HepG2 cells. b The expression level of ANGPTL8 in ANGPTL8 ^−/− and wild-type HepG2 cells. c Effects of ANGPTL8 deficiency on TNFα-induced NF-κB activation in luciferase reporter assays (n = 3). d Effects of ANGPTL8 deficiency on TNFα-induced IL8, CXCL2 and TNFA transcription (n = 3). e Effects of ANGPTL8 deficiency on IFNγ-induced STAT1 transcription (n = 3). f Reconstitution of ANGPTL8-Flag in the ANGPTL8 ^−/− cell lines. g Effects of ANGPTL8 deficiency and reconstitution on TNFα-induced IL8, CXCL2, and TNFA transcription (n = 3). Data are shown as the mean ± SEM, unpaired two-tailed student’s test was used for statistics (c, d, e, g), **p < 0.01, ***p < 0.001, NS > 0.05. Data are representative of three independent experiments