Fig. 6.

Signals promoting the active conformation induce long-range dynamic changes. Backbone amide resonance intensity ratios of active SrcKD states formed upon: a activation loop autophosphorylation, (b) plus dasatinib, binding c plus substrate peptide binding, were analyzed by histograms and by structure mapping (PDB-entry 3G5D, 4YBJ, 4YBK)^30,31 to infer dynamic changes and interpreted as in Fig. 3. Spheres and circles denote split resonances (Supplementary Table 3). Activation loop autophosphorylation causes long-range sharpening and broadening affecting regulatory sites. Dasatinib induces additional sharpening to activation loop autophosphorylation in some regions, while in other regions it sharpens regions previously broadened by activation loop autophosphorylation. Peptide binding induces overall broadening, reverting the sharpening effects induced by dasatinib. Some intensity ratios for the second split resonance (circle) were omitted because of overlap or for clarity because its intensity ratio is similar to the first resonance in terms of magnitude as shown in the key