Fig. 8.

The SH3–SH2 domains interact with the dasatinib stabilized kinase domain of a Src3D fusion construct. Histogram and structure mapping analysis of CSPs between isolated SrcKD•dasatinib and SrcKD•dasatinib from Src3D fusion•dasatinib, suggest an interaction between the regulatory domains and the kinase domain in the context of Src3D fusion•dasatinib. The CSPs were mapped to the structure of SrcKD•dasatinib (PDB: 3G5D) with the regulatory SH3–SH2 domains from near full length Src (PDB: 2SRC PDB) superimposed, indicating that they interact with the SH3 and SH2-binding interfaces. As shown in the histogram the SH3-binding interface comprises of the SH2-SrcKD linker, β2-β3 loop, the SH2-binding site comprises of: helix-E, β7-β8 loop, helix-αI, and the carboxy-terminal tail