Fig. 4.

Experimental demonstration of aberration correction for a target underneath a rat brain tissue. a Layout of the sample geometry. A thick rat brain tissue slice was placed on the top of the resolution target. b, c CLASS images of a resolution target under a 500 μm-thick rat brain tissue layer before and after aberration correction, respectively. Scale bar, 4 μm. Color bars, intensity normalized by the maximum intensity in (b, d and e) Input and output phase correction maps, respectively. Scale bar, k ₀ α. Color bars, phase in radians. f Amplitude transfer function calculated by cross-correlation between (d, e). Scale bar, k ₀ α