Table 1.
List of the olive groves showing symptoms of Olive quick decline syndrome (OQDS) and representing the different foci used to collect the olive samples for the identification of Xylella fastidiosa by quantitative PCR (qPCR) (Harper et al.36) and isolation in axenic culture. In each grove, three symptomatic trees were selected and sampled.
| Foci OQDS | Code of the samples | Municipality (Province) | Date of sampling | Results of the qPCR for X. fastidiosa a | Cultured isolate |
|---|---|---|---|---|---|
| APL-1 | WPT 1129 | Minervino di Lecce (Lecce) | May, 2016 | Positive | YES |
| APL-2 | WPT 1130 | Uggiano la Chiesa (Lecce) | May, 2016 | Positive | YES |
| APL-3 | WPT 1133 | Cursi (Lecce) | May, 2016 | Positive | YES |
| APL-4 | WPT 1134 | Supersano (Lecce) | May, 2016 | Positive | YES |
| APL-5 | WPT 1135 | Maglie (Lecce) | May, 2016 | Positive | YES |
| APL-6 | WPT 1140 | Muro Leccese (Lecce) | June, 2016 | Positive | YES |
| APL-7 | WPT 1141 | Palmariggi (Lecce) | June, 2016 | Positive | YES |
| APL-8 | WPT 1144 | Spongano (Lecce) | June, 2016 | Positive | YES |
| APL-9 | WPT 1145 | Andrano (Lecce) | June, 2016 | Positive | YES |
| APL-10 | WPT 1148 | Tricase (Lecce) | June, 2016 | Positive | YES |
| APL-11 | AS | Cutrofiano (Lecce) | June, 2016 | Positive | YES |
| APL-12 | AV | Avetrana (Taranto) | June, 2016 | Positive | YES |
| APL-13 | CS | Campi Salentina (Lecce) | June, 2016 | Positive | YES |
| APL-14 | CIST | Alliste (Lecce) | May, 2016 | Positive | YES |
| APL-15 | CUT | Cutrofiano (Lecce) | June, 2016 | Positive | YES |
| APL-16 | FP | Presicce (Lecce) | June, 2016 | Positive | YES |
| APL-17 | GC | Gagliano del capo (Lecce) | June, 2016 | Positive | YES |
| APL-18 | GD | Gagliano del capo (Lecce) | June, 2016 | Positive | YES |
| APL-19 | Gigante | Alliste (Lecce) | May, 2016 | Positive | YES |
| APL-20 | Giug | Giuggianello (Lecce) | May, 2016 | Positive | YES |
| APL-21 | La Castellana | Matino (Lecce) | June, 2016 | Positive | YES |
| APL-22 | San CAS | San Cassiano (Lecce) | May, 2016 | Positive | YES |
| APL-23 | TK | Nociglia (Lecce) | May, 2016 | Positive | YES |
| APL-24 | SP1 | Morciano di leuca (Lecce) | June, 2016 | Positive | YES |
| APL-25 | SP3 | Salve (Lecce) | June, 2016 | Positive | YES |
| APL-26 | SP4 | Presicce (Lecce) | June, 2016 | Positive | YES |
| APL-27 | SP7 | Specchia (Lecce) | June, 2016 | Positive | YES |
| APL-28 | Dedonno (CFBP 8402) | Gallipoli (Lecce) | June, 2014 | Positive | YES |
| APL29 | SZ | Squinzano (Lecce) | May, 2016 | Positive | YES |
| APL-30 | TR | Alliste (Lecce) | May, 2016 | Positive | YES |
| APL-31 | UG | Ugento (Lecce) | May, 2016 | Positive | YES |
| APL-32 | ORIA | Oria (Brindisi) | June, 2016 | Positive | YES |
| APL-33 | VEG | Veglie (Lecce) | June, 2016 | Positive | YES |
| APL-34 | CU | Cutrofiano (Lecce) | June, 2016 | Positive | YES |
| APL-35 | FO | Taviano (Lecce) | June, 2016 | Positive | YES |
| APL-36 | VN | Gallipoli (Lecce) | June, 2016 | Positive | YES |
| APL-37 | ST | Sternatia (Lecce) | June, 2016 | Positive | YES |
| APL-38 | GA | Gagliano del capo (Lecce) | June, 2016 | Positive | YES |
| APL-39 | MELC A | Ugento (Lecce) | August, 2014 | Positive | YES |
| APL-40 | COP | Copertino (Lecce) | August, 2014 | Positive | YES |
| APL-41 | CUR | Cursi (Lecce) | August, 2014 | Positive | YES |
| APL-42 | SC | Presicce (Lecce) | August, 2014 | Positive | YES |
| APL-43 | LI SAULI | Gallipoli (Lecce) | June, 2016 | Positive | YES |
| APL-44 | WPT 1137 | Salve (Lecce) | May, 2016 | Positive | YES |
| APL-45 | WPT 1139 | Specchia (Lecce) | May, 2016 | Positive | NO |
| APL-46 | WPT 1142 | Otranto (Lecce) | June, 2016 | Positive | YES |
| APL-47 | WPT 1143 | San Cassiano (Lecce) | June, 2016 | Positive | YES |
| APL-48 | WPT 1146 | Specchia (Lecce) | June, 2016 | Positive | NO |
| APL-49 | WPT 1147 | Alessano (Lecce) | June, 2016 | Positive | NO |
| APL-50 | SP2 | Salve (Lecce) | June, 2016 | Positive | NO |
| APL-51 | SP5 | Miggiano (Lecce) | June, 2016 | Positive | NO |
| APL-52 | SP6 | Montesano Salentino (Lecce) | June, 2016 | Positive | NO |
| APL-53 | SP8 | Specchia (Lecce) | June, 2016 | Positive | NO |
| APL-54 | FP | Presicce (Lecce) | June, 2016 | Positive | YES |
| APL-55 | RAC | Racale (Lecce) | June, 2016 | Positive | YES |
| APL-56 | TR | Trepuzzi (Lecce) | June, 2016 | Positive | YES |
| APL-57 | SQ1 | San Vito dei Normanni (Brindisi) | January, 2017 | Positive | YES |
| APL-58 | SQ2 | Carovigno (Brindisi) | January, 2017 | Positive | YES |
aSamples were assessed as “Positive” when qPCR reactions produced quantitative cycle (Cq) > 0 and < 32; “Doubtful” with Cq > 32; “Negative” when no fluorescence was detected in the reaction, Cq = 0.