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. 2017 Dec 18;7:17748. doi: 10.1038/s41598-017-17669-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CAR T cell transduction and characterization. (a) Scatter plot showing the pure population of human T cells (CD3) after magnetic assisted cell sorting of blood samples. (b) Isolated T cell flow analysis for expression of CD4/CD8 shows that 2/3 of T cells are CD4+ and 1/3 are CD8+. (c) CAR T cell population 2 weeks after transduction shows 85% CAR-expressing T cells. (d) ¹⁹F NMR spectrum showing PFC uptake of CAR T cells (peak at −91 ppm, 2 × 10¹¹ atoms/cell) normalized to the TFA reference (peak at −76 ppm). (e) Flow cytometry histogram showing similar repartition of CD4+ and CD8+ CAR T cells after transduction compared to untransduced T cells (b). (f) CAR T cells labeled with PFC ex vivo exhibit comparable phenotype to unlabeled cells.