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. 2017 Dec 18;7:17748. doi: 10.1038/s41598-017-17669-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Human CAR T cell cytotoxicity assay in vitro. Co-incubation of CAR T cells or untransduced T cells with human U87-EGFRvIII-Luc glioma cells resulted in significant cell death at 6, 12 and 24 h. CAR T cells exhibit significant tumor killing ability (93%) compared to untransduced T cells (53%, p < 0.0001 indicated by *). The relative glioma cell cytotoxicity was obtained from the mean photon count, and the ratio of treated versus untreated means are displayed as percentages ± standard error.