Table 1.
Composition of mediaz used for regeneration of Ensete ventricosum (Welw.) Cheesman (enset)
| Name of medium | Ascorbic acid (10 mg L−1) | BAP (mg L−1) | 2,4-D (mg L−1) | Zeatin (mg L−1) | Activated charcoal (2 g L−1) |
|---|---|---|---|---|---|
| RM1 | Yes | 1.0 | 0.0 | 0.0 | No |
| PM1 | Yes | 2.5 | 0.0 | 0.0 | Yes |
| PM2 | Yes | 1.0 | 0.0 | 0.0 | Yes |
| PM3 | Yes | 5.0 | 0.0 | 0.0 | Yes |
| PM4 | Yes | 1.0 | 0.0 | 0.0 | No |
| PM5 | Yes | 2.5 | 0.0 | 0.0 | No |
| PM6 | Yes | 5.0 | 0.0 | 0.0 | No |
| CIM1 | Yes | 0.0 | 1.5 | 0.216 | Yes |
| CIM2 | Yes | 0.0 | 1.5 | 0.216 | No |
| MBM1 | Yes | 0.0 | 0.0 | 0.0 | Yes |
| MBM2 | Yes | 0.0 | 0.0 | 0.0 | No |
All chemicals purchased from Duchefa Biochemie, Haarlem, Netherlands.
RM regeneration medium, PM proliferation medium, CIM callus induction medium, MBM MS basal medium, 2,4-D 2,4-dichlorophenoxyacetic acid, BAP 6-benzylaminopurine
zIn addition to components shown in the table, all media contain Murashige and Skoog (1962) salts and vitamins, 30 g L−1 sucrose, and 2 g L−1 Gelrite™, and are adjusted to pH 5.8 prior to autoclaving.