Table 3.
Multiple bud induction medium | BAP (mg L−1) | Activated charcoal (2 g L−1) | Efficiency of bud induction at 4 wk (%)za | Shoot regeneration medium | BAP (mg L−1) | Activated charcoal (2 g L−1) | Number of shoots initiated per corm disca | Number of complete plants per corm disca | Multiplication rateya | |
---|---|---|---|---|---|---|---|---|---|---|
1 | PM2 | 1.0 | Yes | 81.7 ± 14.1 | PM2 | 1.0 | Yes | 29.6 ± 1.6 | 12.0 ± 0.9 | 36.1 ± 2.8 |
2 | PM1 | 2.5 | Yes | 71.0 ± 10.2 | PM1 | 2.5 | Yes | 26.3 ± 2.9 | 4.6 ± 0.5 | 13.7 ± 1.5 |
3 | PM3 | 5.0 | Yes | 73.3 ± 11.6 | PM3 | 5.0 | Yes | 33.5 ± 8.9 | 4.3 ± 0.9 | 13.0 ± 2.8 |
4 | PM4 | 1.0 | No | 68.7 ± 10.3 | PM4 | 1.0 | No | 0 ± 0 | 0 ± 0 | 0 ± 0 |
PM2 | 1.0 | Yes | 28.4 ± 3.9 | 0.9 ± 0.1 | 2.8 ± 0.4 | |||||
5 | PM5 | 2.5 | No | 75.3 ± 4.0 | PM5 | 2.5 | No | 0 ± 0 | 0 ± 0 | 0 ± 0 |
PM1 | 2.5 | Yes | 28.7 ± 4.1 | 0.4 ± 0.1 | 1.1 ± 0.3 | |||||
6 | PM6 | 5.0 | No | 64.0 ± 3.5 | PM6 | 5.0 | No | 0 ± 0 | 0 ± 0 | 0 ± 0 |
PM3 | 5.0 | Yes | 47.4 ± 4.3 | 0.7 ± 0.1 | 2.1 ± 0.2 | |||||
7 | MBM1 | 0.0 | Yes | 55.3 ± 4 | MBM1 | 0.0 | Yes | 42.3 ± 2.9 | 11.6 ± 0.36 | 34.9 ± 1.1 |
8 | MBM2 | 0.0 | No | 50.7 ± 4 | MBM2 | 0.0 | No | 30.6 ± 0.7 | 1.8 ± 0.2 | 5.3 ± 0.6 |
aValues are mean ± Std.
zEfficiency of bud induction (%) = (number of explants producing multiple buds / total number of explants cultured) × 100.
yMultiplication rate = total number of plantlets regenerated from three corm discs isolated from one in vitro mother plantlet.