Table 2.
Primers Used for Real-Time PCR
| Genes | Species | Sense (5′–3′) | Antisense (5′–3′) |
|---|---|---|---|
| RHEB | Hum | GGAATCTTCTGCTAAAGAAA | GCATGAAGACTTGCCTTGTG |
| RICTOR | Hum | AGTGAATCTGTGCCATCGAG | AAGGAAAAATGTGCTAGTAGAGCT |
| α-SMA | Hum | GGGAATGGGACAAAAAGACA | CTTCAGGGGCAACACGAA |
| COLL1A1 | Hum | GGGATTCCCTGGACCTAAAG | GGAACACCTCGCTCTCCA |
| COLL1A2 | Hum | CTGGAGAGGCTGGTACTGCT | AGCACCAAGAAGACCCTGAG |
| COLL3A1 | Hum | CTGGACCCCAGGGTCTTC | GACCATCTGATCCAGGGTTTC |
| β-actin | Mus | CTAAGGCCAACCGTGAAAAG | ACCAGAGGCATACAGGGACA |
| RHEB | Mus | TTTTTGGAATCTTCTGCTAAAGAAA | TGAAGCTGCTCCATCAATCTT |
| RICTOR | Mus | ACATTCAGCAGAGCAACGAG | GGAAACAAGGAAGCATTCACA |
| COLL1A1 | Mus | TTCTCCTGGCAAAGACGGAC | CGGCCACCATCTTGAGACTT |
| COLL1A2 | Mus | TAGCCAACCGTGCTTCTCAG | TCTCCTCATCCAGGTACGCA |
| COLL3A1 | Mus | AAGGCTGCAAGATGGATGCT | GTGCTTACGTGGGACAGTCA |
| α-SMA | Mus | GAGACTCTCTTCCAGCCATCTT | TGATCTCCTTCTGCATCCTGTC |
Hum, human; Musc, mouse.