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Agarose gel electrophoresis of crude DNA (D1 & E1) and universal PCR (D2 & E2) using universal primer set 27f and 1525r. D1 & D2: Procedure B (Gabor harsh). E1 & E2: Procedure C (Gabor soft). Lane declaration for all crude DNA and universal PCR gel images: lane 1 to 3 activated sludge; lane 4 to 6 Havel River sediment; lane 7 to 9 anaerobic digestion sludge; lane 10 to 12 nitrifying sludge; M in all gel images: 10 kb MassRuler DNA ladder.
