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Agarose gel electrophoresis of crude DNA (F1 & G1) and universal PCR (F2 & G2) using universal primer set 27f and 1525r. F1 & F2: Procedure D (Shan). G1 & G2: Procedure E (Orsini & Romano-Spica). Lane declaration for all crude DNA and universal PCR gel images: lane 1 to 3 activated sludge; lane 4 to 6 Havel River sediment; lane 7 to 9 anaerobic digestion sludge; lane 10 to 12 nitrifying sludge; M in all gel images: 10 kb MassRuler DNA ladder.
