Table 2.
Cost analysis for DNA extraction procedure A (according to Bourrain et al., 1999).
| Consumables | Volumes | Units | Concentration | Volumes | /Weight |
High costs |
Low costs |
Low cost | High cost | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Amount | Unit | Fix cost (€) | Amount | Unit | Fix cost (€) | per prep (€) | per prep (€) | ||||||
| Gloves (any size) | 1 | pair | – | – | – | 50 | pair | 8.20 | 50 | pair | 4.50 | 0.090 | 0.1640 |
| Tubes | 5 | – | – | 2.0 | mL | 500 | pieces | 11.9 | 1000 | pieces | 21.90 | 0.1095 | 0.1190 |
| Tips | 12 | – | – | 1000 | µL | 500 | pieces | 5.08 | 1000 | pieces | 7.70 | 0.0924 | 0.1218 |
| Tips | 1 | – | – | 200 | µL | 500 | pieces | 5.40 | 1000 | pieces | 8.19 | 0.0082 | 0.0108 |
| Lysozyme buffer | 0.75 | mL | 0.15 M NaCl | 6,6 | mg | 500 | g | 15.84 | 1000 | g | 24.19 | 0.0002 | 0.0002 |
| 0.1 M Na2EDTA | 27.9 | mg | 100 | g | 23.50 | 1000 | g | 59.70 | 0.0017 | 0.0066 | |||
| 15 mg mL-1 Lysozyme | 15.0 | mg | 1.0 | g | 23.89 | 10 | g | 96.04 | 0.1441 | 0.3584 | |||
| SDS solution | 0.75 | mL | 0.1 M NaCl | 4.4 | mg | 500 | g | 15.84 | 1000 | g | 24.19 | 0.0001 | 0.0001 |
| 0.5 M Tris–HCl | 45.4 | mg | 500 | g | 93.40 | 1000 | g | 128.00 | 0.0058 | 0.0085 | |||
| w/v 10% SDS | 0.075 | mg | 100 | g | 16.56 | 1000 | g | 56.48 | 0.0000 | 0.0000 | |||
| Tris–HCl saturated phenol | 1.0 | mL | 0.1 M Tris–HCl | 12.1 | mg | 500 | g | 93.40 | 1000 | g | 128.00 | 0.0016 | 0.0023 |
| Phenol | 1.0 | g | 100 | g | 18.00 | 1000 | g | 64.40 | 0.0644 | 0.1800 | |||
| Phenol:Chloroform:Isoamyl | 1.0 | mL | 25′ Phenol | 0.5 | g | 100 | g | 18.00 | 1000 | g | 64.40 | 0.0322 | 0.0900 |
| (25:24:1 v/v) | 24′ Chloroform | 0.48 | mL | 500 | mL | 50.62 | 2500 | mL | 100.66 | 0.0193 | 0.0486 | ||
| 1′ Isoamyl | 0.02 | mL | 25 | mL | 13.92 | 1000 | mL | 108.00 | 0.0022 | 0.0111 | |||
| Chloroform:Isoamyl | 1.0 | mL | 24′ Chloroform | 0.96 | mL | 500 | mL | 50.62 | 2500 | mL | 100.66 | 0.0387 | 0.0972 |
| (24:1 v/v) | 1′ Isoamyl | 0.04 | mL | 25 | mL | 13.92 | 1000 | mL | 108.00 | 0.0043 | 2.2E-05 | ||
| Isopropanol | 1.0 | mL | 100% | 2.0 | mL | 1000 | mL | 30.30 | 2500 | mL | 61.70 | 0.0494 | 6.1E-02 |
| TE buffer | 0.1 | mL | 10 mM Tris–HCl | 0.12 | mg | 500 | g | 93.40 | 1000 | g | 128.00 | 1.6E-05 | 2.3E-05 |
| 1 mM EDTA | 0.03 | mg | 100 | g | 34.08 | 1000 | g | 245.23 | 7.2E-06 | 1.0E-05 | |||
| RNaseA treatment | 5.0 | µL | 0.2 µg µL-1 | 1.0 | µg | 250 | mg | 94.40 | 1000 | mg | 292.00 | 0.0003 | 3.8E-04 |
| Extracted samples | 12 | – | – | – | – | – | – | ||||||
| Extraction time | 250 | min | – | – | – | – | – | – | |||||
| Lab staff (per hour) | – | 35.00 | – | 25.00 | – | – | |||||||
| Lab staff (€/extraction) | 8.68 | 12.15 | |||||||||||
| Chemicals (€/extraction) | 0.36 | 0.86 | |||||||||||
| Gloves, tubes, tips (€/extraction) | 0.30 | 0.42 | |||||||||||
| Final price per extraction including extraction time, lab staff and all consumables (€) | 9.34 | 13.43 | |||||||||||