Table 4.

Cost analysis for DNA extraction procedure C (according to Gabor et al. [2]; soft method).

Consumables	Volumes	Units	Concentration	Volumes	/Weight	High costs			Low costs			Low cost	High Cost
						Amount	Unit	Fix cost (€)	Amount	Unit	Fix cost (€)	per Prep (€)	per Prep (€)
Gloves (any size)	1	pair	–	–	–	50	pair	8.20	50	pair	4.50	0.090	0.164
Tubes	3	–	–	2.0	mL	500	pieces	11.90	1000	pieces	21.90	0.066	0.071
Tips	10			1000	µL	500	pieces	5.08	1000	pieces	7.70	0.077	0.102
Tips	4			200	µL	500	pieces	5.40	1000	pieces	8.19	0.033	0.043
Tips	1			10	µL	1000	pieces	27.14	2000	pieces	43.42	0.022	0.027
Silica beads	0.1	mm	ID	700	mg	1000	g	24.30	25000	g	202.00	0.0057	0.0170
Lysozyme buffer	1.25	mL	100 mM Tris	15.1	mg	500	g	93.40	1000	g	128.00	0.0019	0.0028
		mL	100 mM sodium EDTA	46.5	mg	100	g	23.50	1000	g	59.70	0.0028	0.0109
			100 M NaCl	109.6	mg	500	g	15.84	1000	g	24.19	0.0027	0.0035
			1% w/v CTAB	12.5	µg	100	g	22.64	1000	g	89.11	0.0011	0.0028
Lysozyme	0.04	mL	50 mg mL-1	2.0	mg	1.0	g	23.89	10	g	96.04	0.0192	0.0478
Proteinase K	0.01	mL	10 mg mL-1	0.1	mg	0.1	g	67.68	0.5	g	259.62	0.0519	0.0677
SDS	0.2	mL	w/v 20%	0.04	mg	100	g	16.56	1000	g	56.48	2.3E-06	6.6E-06
Chloroform (1:1 v/v)	1.0	mL	100%	1.0	mL	500	mL	50.62	2500	mL	100.66	0.0403	0.1012
Isopropanol (0.6:1 v/v)	0.6	mL	100%	0.6	mL	1000	mL	30.30	2500	mL	61.70	0.0148	0.0182
Ethanol	0.5	mL	70%	0.375	mL	250	mL	47.56	2500	mL	246.58	0.0345	0.0666
TE buffer	0.1	mL	10 mM Tris–HCl	0.12	mg	500	g	93.40	1000	g	128.00	1.6E-05	2.3E-05
			1 mM EDTA	0.03	mg	100	g	34.08	1000	g	245.23	7.2E-06	1.0E-05
Extracted samples	12					–		–	–		–	–	–
Extraction time	230	min				–		–	–		–	–	–
Lab staff (per hour)						–		35.00	–		25.00	–	–
Lab staff (€/extraction)												7.99	11.18
Chemicals (€/extraction)												0.17	0.34
Gloves, tubes, tips (€/extraction)												0.29	0.41
Final price per extraction including extraction time, lab staff and all consumables (€)												8.45	11.93