. 2017 Dec 5;16:732–751. doi: 10.1016/j.dib.2017.11.082

Table 5.

Cost analysis for DNA extraction procedure D (according to Shan et al. [3]).

Consumables	Volumes	Units	Concentration	Volumes	/Weight	High costs			Low costs			Low cost	High cost
Consumables	Volumes	Units	Concentration	Volumes	/Weight	Amount	Unit	Fix cost (€)	Amount	Unit	Fix cost (€)	per Prep (€)	per Prep (€)
Gloves (any size)	1	pair	–	–	–	50	pair	8.20	50	pair	4.50	0.0900	0.1640
Tubes	3	–	–	2.0	mL	500	pieces	11.90	1000	pieces	21.90	0.0657	0.0714
Tips	8			1000	µL	500	pieces	5.08	1000	pieces	7.70	0.0616	0.0812
Tips	2			200	µL	500	pieces	5.40	1000	pieces	8.19	0.0164	0.0216
Tips	1			10	µL	1000	pieces	27.14	2000	pieces	43.42	0.0217	0.0271
TENP Puffer	0.4	mL	50 mM Tris	2.42	mg	500	g	93.40	1000	g	128.00	0.0003	0.0005
			20 mM EDTA	2.34	mg	100	g	34.08	1000	g	245.23	0.0006	0.0008
			100 mM NaCl	2.34	mg	500	g	15.84	1000	g	24.19	0.0001	0.0001
			10 mg mL-1 PVP	4.00	mg	100	g	45.30	1000	g	224.00	0.0009	0.0018
SDS	50	µL	w/v 20%	10.0	µg	10]0	g	16.56	1000	g	56.48	5.6E-07	1.7E-06
CTAB Puffer	0.5	mL	0,7 M NaCl	20.5	mg	500	g	15.84	1000	g	24.19	0.0005	0.0006
			10% CTAB	50.0	µg	100	g	22.64	1000	g	89.11	4.5E-06	1.1E-05
KH₂PO₄	0.25	mL	240 mM	8.16	mg	250	g	19.66	1000	g	56.66	4.6E-07	0.0006
Phenol:Chloroform:Isoamyl	1.0	mL	100 mM Tris	12.1	mg	500	g	93.40	1000	g	128.00	0.0016	0.0023
(25:24:1 v/v)			Phenol	0.50	g	100	g	18.00	1000	g	64.40	0.0322	0.0900
			Chloroform	0.48	mL	500	mL	50.62	2500	mL	100.66	0.0193	0.0486
			Isoamyl	0.02	mL	25	mL	13.92	1000	mL	108.00	0.0022	0.0111
Chloroform:Isoamyl	1.0	mL	Chloroform	0.96	mL	500	mL	50.62	2500	mL	100.66	0.0387	0.0972
(24:1 v/v)			Isoamyl	0.04	mL	25	mL	13.92	1000	mL	108.00	0.0043	0.0223
Isopropanol	1.0	mL	100%	1.0	mL	1000	mL	30.30	2500	mL	61.70	0.0247	0.0303
TE buffer	0.1	mL	10 mM Tris–HCl	0.12	mg	500	g	93.40	1000	g	128.00	1,6E-05	2.3E-05
			1.0 mM EDTA	0.03	mg	100	g	34.08	1000	g	245.23	7.2E-06	1.0E-05
Extracted samples	12					–		–	–			–	–
Extraction time	210	min				–		–	–		–	–	–
Lab staff (per hour)						–		35.00	–		25.00	–	–
Lab staff (€/extraction)												7.29	10.21
Chemicals (€/extraction)												0.13	0.31
Gloves, tubes, tips (€/extraction)												0.26	0.37
Final price per extraction including extraction time, lab staff and all consumables (€)												7.67	10.88