Table 6.
Cost analysis for DNA extraction procedure E (according to Orsini and Romano-Spica [4]).
| Consumables | Volumes | Units | Concentration | Volumes | /Weight |
High costs |
Low costs |
Low cost | High cost | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Amount | Unit | Fix cost (€) | Amount | Unit | Fix cost (€) | per Prep (€) | per Prep (€) | ||||||
| Gloves (any size) | 1 | pair | – | – | – | 50 | pair | 8.20 | 50 | pair | 4.50 | 0.0900 | 0.1640 |
| Tubes | 2 | – | – | 2.0 | mL | 500 | pieces | 11.90 | 1000 | pieces | 21.90 | 0.0438 | 0.0476 |
| Tips | 9 | – | 1000 | µL | 500 | pieces | 5.08 | 1000 | pieces | 7.70 | 0.0693 | 0.0914 | |
| Tips | 3 | – | 200 | µL | 500 | pieces | 5.40 | 1000 | pieces | 8.19 | 0.0246 | 0.0324 | |
| Tips | 0 | – | 10 | µL | 1000 | pieces | 27.14 | 2000 | pieces | 43.42 | 0,0000 | 0.0000 | |
| Wash solution | 1.0 | mL | 50 mM Tris–HCl | 6.1 | mg | 500 | g | 93.40 | 1000 | g | 128.00 | 0.0008 | 0.0011 |
| 25 mM EDTA | 7.3 | mg | 100 | g | 34.08 | 1000 | g | 245.23 | 0.0018 | 0.0025 | |||
| 0.1% w/v SDS | 1.0 | µg | 100 | g | 16.56 | 1000 | g | 56.48 | 5.6E-08 | 1.7E-07 | |||
| 0.1% w/v PVP | 1.0 | µg | 100 | g | 45.30 | 1000 | g | 224.00 | 2.2E-07 | 4.5E-07 | |||
| Lysis buffer | 0.1 | mL | 50 mM Tris–HCl | 0.61 | mg | 500 | g | 93.40 | 1000 | g | 128.00 | 7.8E-05 | 1.1E-04 |
| 25 mM EDTA | 0.73 | mg | 100 | g | 34.08 | 1000 | g | 245.23 | 1.8E-04 | 2.5E-04 | |||
| 3% w/v SDS | 30.0 | µg | 100 | g | 16.56 | 1000 | g | 56.48 | 1.7E-06 | 5.0E-06 | |||
| 1.2% w/v PVP | 12.0 | µg | 100 | g | 45.30 | 1000 | g | 224.00 | 2.7E-06 | 5.4E-06 | |||
| Extraction buffer | 0.8 | mL | 10 mM Tris–HCl | 9.7 | mg | 500 | g | 93.40 | 1000 | g | 128.00 | 0.0012 | 0.0018 |
| 1 mM EDTA | 0.23 | mg | 100 | g | 34.08 | 1000 | g | 245.23 | 0.0001 | 0.0001 | |||
| 0.3 M NaOAc | 19.7 | mg | 250 | g | 22.47 | 1000 | g | 56.30 | 0.0011 | 0.0018 | |||
| 1.2% PVP | 9.6 | µg | 100 | g | 45.30 | 1000 | g | 224.00 | 2.2E-06 | 4.3E-06 | |||
| Phenol:Chloroform | 1.0 | mL | Phenol | 0.5 | g | 100 | g | 18.00 | 1000 | g | 64.40 | 0.0322 | 0.0900 |
| (1:1 v/v) | Chloroform | 0.5 | mL | 500 | mL | 50.62 | 2500 | mL | 100.66 | 0.0201 | 0.0506 | ||
| Sodiumacetate | 0.08 | mL | 3 M | 19.7 | mg | 250 | g | 22.47 | 1000 | g | 56.30 | 0.0011 | 0.0018 |
| Isopropanol | 0.9 | mL | 100% | 0.9 | mL | 1000 | mL | 30.30 | 2500 | mL | 61.70 | 0.0222 | 0.0273 |
| Ethanol | 2.0 | mL | 70% | 1.4 | mL | 250 | mL | 47.56 | 2500 | mL | 246.58 | 0.1381 | 0.2663 |
| TE buffer | 0.1 | mL | 10 mM Tris–HCl | 0.12 | mg | 500 | g | 93.40 | 1000 | g | 128.00 | 1.6E-05 | 2.3E-05 |
| 1.0 mM EDTA | 0.03 | mg | 100 | g | 34.08 | 1000 | g | 245.23 | 7.2E-06 | 1.0E-05 | |||
| Extracted samples | 12 | – | – | – | – | – | – | ||||||
| Extraction time | 150 | min | – | – | – | – | – | – | |||||
| Lab staff (per hour) | – | 35.00 | – | 25.00 | – | – | |||||||
| Lab staff (€/extraction) | 5.21 | 7.29 | |||||||||||
| Chemicals (€/extraction) | 0.22 | 0.44 | |||||||||||
| Gloves, tubes, tips (€/extraction) | 0.23 | 0.34 | |||||||||||
| Final price per extraction including extraction time, lab staff and all consumables (€) | 5.65 | 8.07 | |||||||||||